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低浓度玉米小斑病菌C小种毒素培养滤液诱导寄主抗性的研究

DOI: 10.7668/hbnxb.2008.02.044, PP. 198-204

Keywords: 玉米小斑病菌C小种,毒素培养滤液,苯丙氨酸解氨酶(PAL),过氧化物酶(POD),丙二醛(MDA),诱导抗性

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Abstract:

利用低浓度玉米小斑病菌C小种毒素培养滤液作为激发子诱导玉米获得抗病性。以一对同核异质玉米B37自交系为试材,采用离体叶片接种法检测适宜诱导抗性的低浓度玉米小斑病菌C小种毒素培养滤液作为激发子诱导玉米抗病性的适宜诱导浓度,测定与抗病性相关酶的变化。经用1∶50和1∶60的低浓度玉米小斑病菌C小种毒素培养滤液预处理植株,再接种高浓度(1∶10)毒素培养滤液处理的病斑面积分别为(0.30±0.14)和(0.36±0.17)mm2,而对照为(2.70±0.24)mm2,是处理的7.5~9倍,差异极显著。经0~72h的动态检测,以1∶60预处理效果为最佳,与对照相比,苯丙氨酸解氨酶(PAL)活性平均提高了23.4%,过氧化物酶(POD)活性平均提高了65.9%,有害物质丙二醛(MDA)的含量平均降低了28.2%。叶片内部所产生的生化反应与叶片表面的抗性病理反应相吻合,证实低浓度玉米小斑病菌C小种毒素滤液本身能够成功地作为激发子来诱导寄主-玉米获得抗性。

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