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根癌农杆菌介导的番茄高效遗传转化体系的研究

DOI: 10.7668/hbnxb.2009.02.015, PP. 71-74

Keywords: 农杆菌介导法,遗传转化,番茄

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Abstract:

以番茄无菌苗的子叶和下胚轴为外植体,通过农杆菌介导法,对其遗传转化条件进行了优化,建立了高效番茄子叶和下胚轴农杆菌介导的遗传转化体系.结果表明:在农杆菌浸染前(浸染浓度为OD600=0.3)进行2d的预培养浸染6min的外植体在MS+210mg/L6-BA+015mg/LIAA的培养基上转化效率最高.PCR检测初步证明,NPTII基因已整合到番茄再生植株中.

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