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柞蚕核型多角体病毒p11基因克隆及序列分析

DOI: 10.7668/hbnxb.2009.02.009, PP. 42-46

Keywords: 杆状病毒,p11基因,序列分析

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Abstract:

为丰富柞蚕核型多角体病毒分子生物学基础,通过PCR扩增克隆了ApNPVp11基因并进行了序列的生物信息学分析.ApNPVp11基因编码102个氨基酸,预测分子量11.2kDa;氨基酸序列N端1~33位是信号肽序列,中部0~72位是跨膜区.PSI-BLAST搜索表明有20种核型多角体病毒编码蛋白与ApNPVP11蛋白有显著性匹配;比对分析发现P11蛋白氨基酸序列中部相对保守,两端变异较大.进化分析表明ApNPV属于NPV类群Ⅰ且与EppoNPV、AgMNPV、CfDefNPV亲缘关系较近.

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