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鸡猪源大肠杆菌CTX-M型ESBLs的分子检测

DOI: 10.7668/hbnxb.2009.02.019, PP. 90-93

Keywords: 大肠杆菌,耐药,CTX-M

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Abstract:

为了解河南地区鸡猪源CTX-M型超广谱β-内酰胺酶(ESBLs)的流行情况,并进行基因型分析,通过表型确证试验检测产ESBLs大肠杆菌,分别用5组CTX-M型引物对其进行聚合酶链式反应扩增.表型确证试验检测结果显示,鸡、猪源大肠杆菌中产ESBLs菌株检出率分别为62.8%(59/9)和78.3%(36/6).PCR扩增结果进一步显示,鸡源大肠杆菌中CTX-M型ESBLs阳性率为89.8%(53/59),其中CTX-M-2组型1株,CTX-M-9组型26株,CTX-M-2和CTX-M-9组型13株;猪源大肠杆菌CTX-M型ESBLs的阳性检出率为69.%(25/36),其中,CTX-M-2组型7株,CTX-M-9组型17株,CTX-M-2和CTX-M-9组型1株.河南地区鸡、猪源大肠杆菌CTX-M型ESBLs的产生率较高,而以CTX-M-2组型和CTX-M-9组型为主.

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