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不同饲养条件下内蒙古绒山羊瘤胃甲烷菌组成的研究

DOI: 10.7668/hbnxb.2010.05.047, PP. 228-233

Keywords: 甲烷菌,mcrA基因,瘤胃液,绒山羊

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Abstract:

为了解天然放牧和舍饲条件下内蒙古绒山羊瘤胃中甲烷菌的种群组成.选取甲烷菌mcrA基因的特异性引物序列,利用PCR技术对从瘤胃液中抽提的细菌总DNA进行扩增,并建立了甲烷菌特异性的mcrA基因文库.用限制性内切酶TaqI对该文库特异性片段进行了限制性酶切片断长度多态性分析(Restrictionfragmentlengthpolymorphism,RFLP).放牧和舍饲内蒙古绒山羊瘤胃中甲烷菌的mcrA基因片段被分成个不同的RFLP类型,并由此建立了DNA指纹图谱;系统发育树分析表明,其属于甲烷杆菌目和甲烷微菌目,且绒山羊瘤胃中的甲烷菌大部分属于甲烷短杆菌.放牧绒山羊瘤胃内甲烷菌种类比舍饲绒山羊更加丰富,种甲烷菌所占比例均较高,而舍饲绒山羊只有两种优势菌比例较高,其他几种所占比例极低.

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