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河北省棉花黄萎菌落叶型和非落叶型菌系初步鉴定

DOI: 10.7668/hbnxb.2010.04.041, PP. 196-200

Keywords: 棉花黄萎菌,落叶型菌系,非落叶型菌系,初步鉴定

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Abstract:

利用PCR特异性扩增技术对从采自河北省的228株棉花黄萎菌(Verticilliumdahliae)、四川省的2株棉花黄萎菌(菌株Sch-1和Sch-3)、辽宁省的1株黄萎菌(菌株Ly-1)、棉花黄萎菌标准菌株(落叶型标准菌株T9和非落叶型标准菌株V97)和3株棉花枯萎菌(Fusariumoxysporumf.sp.vasinfectum)菌株进行了落叶型和非落叶型菌系的鉴定.结果表明,利用V.dahliae特异性引物DB19/DB22从233株黄萎菌中均可获得00bp的特异性片段,而3株棉花枯萎菌中不能获得扩增产物.分别利用非落叶型特异性引物INTNDf/INTNDr和落叶型特异性引物INTD2f/INTD2r对233株黄萎菌进行PCR扩增.菌株Sch-1、Sch-3、Ly-1及非落叶型黄萎菌标准菌株V97利用非落叶型特异性引物INTNDf/INTNDr可扩增出1100bp的产物;采自河北省的228株黄萎菌和落叶型标准菌株T9利用落叶型特异性引物INTD2f/INTD2r可扩增出40bp的产物.结果表明,目前河北省主要棉区棉花黄萎病菌以落叶型黄萎菌菌株为主.

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