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CaM及各亚型基因参与小麦抗叶锈病反应的研究

DOI: 10.7668/hbnxb.2010.04.037, PP. 175-179

Keywords: 小麦,叶锈菌,钙调素,亚型,实时荧光定量PCR

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Abstract:

采用实时荧光定量PCR法,对小麦抗叶锈病近等基因系TcLr1接种亲和或非亲和性叶锈菌后,CaM及其亚型的mRNA表达差异进行相对定量分析.结果表明,小麦接菌后12h内CaM的4个亚型基因表达量与不接菌对照相比相差不大.小麦接种非亲和叶锈菌,48,72,96h后,CaMSF-1表达量分别比亲和组合高24.6%,26.6%,38.8%;接种24,48h后,CaMSF-4表达量分别高出亲和组合26.8%和28.0%;在接种后24~96h这一过程中,CaMSF-2表达量均低于亲和组合中,而CaMSF-3表达量与亲和组合表达量相差不大.接种非亲和叶锈菌后,在第24h、第48h小麦CaM表达量分别高于亲和组合18.1%和47.9%,而在第72h和第96hCaM表达量又低于亲和组合.上述结果暗示,CaM可能参与了小麦抗叶锈病反应,并且具有亚型特异性.小麦中CaMSF-1和CaMSF-4可能与小麦抗叶锈病相关,CaMSF-2可能与小麦感叶锈病相关,而CaMSF-3可能不参与小麦抗叶锈病反应.

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