全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

陕西省地方小麦品种黄色素含量基因的遗传分析

DOI: 10.7668/hbnxb.2010.04.018, PP. 83-87

Keywords: 普通小麦,黄色素,八氢番茄红素合成酶基因,功能标记,分子标记辅助选择

Full-Text   Cite this paper   Add to My Lib

Abstract:

面粉及其制成品的色泽是衡量小麦品质的重要指标之一.八氢番茄红素合成酶(Phytoenesynthase,Psy)基因是影响小麦黄色素含量的关键基因,利用分子标记检测98份陕西省地方小麦品种(系)Psy-A1与Psy-B1基因的等位变异及其分布,进一步验证Psy-A1与Psy-B1基因分子标记的有效性.利用黄色素含量基因(Psy-A1、Psy-B1)的功能标记YP7A和YP7B检测了98份陕西省1980年以来自育和引进的小麦品种(系)Psy-A1等位基因Psy-A1a(片段长度为194bp,高黄色素含量)、Psy-A1b(片段长度为231bp,低黄色素含量)和Psy-B1等位基因Psy-B1a(片段长度为11bp,中黄色素含量)、Psy-B1b(片段长度为16bp,低黄色素含量)、Psy-B1c(片段长度为428bp,高黄色素含量)与Psy-B1d(片段长度为884bp,不确定与黄色素含量的关系)的分布情况,并探讨了Psy-A1、Psy-B1等位基因在品种间的遗传关系.结果表明,陕西省主栽小麦品种(系)中Psy-A1a和Psy-A1b基因型的频率分别为39.8%和60.2%,以低黄色素含量的Psy-A1b基因型为主;Psy-B1a、Psy-B1b、Psy-B1c与Psy-B1d四种基因型的分布频率分别是24.%,67.4%,7.1%和1.0%.对属于黄淮冬麦区之陕西汾渭河谷副区的小麦品种(系)Psy-A1a、Psy-A1b、Psy-B1a、Psy-B1b、Psy-B1c和Psy-B1d基因型的分布频率分别为4.7%、4.3%,17.4%,71.7%,10.9%和0%;对属于西南冬麦区之陕南鄂西丘陵副区的小麦品种(系)Psy-A1a、Psy-A1b、Psy-B1a、Psy-B1b、Psy-B1c和Psy-B1d基因型的分布频率则分别为32.7%,67.3%,30.8%,63.%,3.9%和1.9%,说明不同副麦区的小麦品种(系)基因型差异明显,同时可以直接利用黄色素含量基因(Psy-A1和Psy-B1)的功能标记YP7A和YP7B来提高小麦育种效率.

References

[1]  Pozniak C J,Knox R E,Clarke F R,et al.Identification of QTL and association of a phytoene synthase gene with endosperm color in durum wheat[J].Theor Appl Gene,2007,114:525-537.
[2]  He X Y,Zhang Y L,He Z H,et al.Characterization of a phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker.Theor Appl Genet,2008,116:213-221.
[3]  He X Y,He Z H,Ma W,et al.Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour color[J].Mol Breeding,2009,23:553 563.
[4]  温彩虹,李酶.陕西小麦良种繁育体系建设的思考[J].陕西农业科学,2007(1):18-19.
[5]  陈雪燕.陕西省小麦地方品种遗传多样性研究[D].硕士研究生论文,2007:p3.
[6]  Lagudah E S,Appels R,McNeil D.The Nor-D3 locus of Triticum tauschii:natural variation and genetic linkage to markers in chromosome 5[J].Genome,1991,34:387-395.
[7]  杨芳萍,何心尧,何中虎,等.中国小麦品种黄色素含量基因等位变异分子检测及其分布规律研究[J].中国农业科学,2008,41(10):2923-2930.
[8]  葛秀秀,何中虎,杨金,等.我国冬小麦品种多酚氧化酶活性的遗传变异及其与品质性状的相关分析[J].作物学报,2003,29:481-485.
[9]  葛秀秀,张立平,何中虎.冬小麦PPO 活性的主基因+多基因混合遗传分析.作物学报[J],2004,30:18-20.
[10]  He Z H,Yang J,Zhang Y,et al.Pan bread and dry white Chinese noodle quality in Chinese winter wheats[J].Euphytica,2004,139:257-267.
[11]  Lin Z J,Miskelly D M,Moss H J.Suitability of various Australian wheat for Chinese-style steamed bread[J].Journal of t he Science of Food and Agriculture,1990,53:203-213.
[12]  杨金,张艳,何中虎.小麦品质性状与面包和面条品质关系分析[J].作物学报,2004,30(8):739-744.
[13]  Miskelly D M.Flour components affecting paste and noodle color[J].Journal of t he Science of Food and Agriculture,1984,35:463-471.
[14]  Adom K K,Sorrells M E,Liu R H.Phytochemical profiles and antioxidant activity of wheat varieties[J].Journal of Agricultural and Food Chemistry,2003,51:7825-7834.
[15]  Kumagai M H,Donson J,Della-Cioppa G,et al.Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA[J].Proceedings of the National Academy of Sciences of the United States of America,1995,92:1679-1683.
[16]  Von Lintig J,Welsch R,Bonk M,et al.Light-dependent regulation of carotenoid biosynthesis occurs at the level of phytoene synthase expression and is mediated by phytochrome in Sinapis alba and Arabidopsis thaliana seedlings[J].The Plant Journal,1997,12:625-634.
[17]  He X Y,He Z H,Ma W,et al.Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour color[J].Molecular Breeding,2009,23(4):553-563.
[18]  Baik B K,Czuchajowska Z,Pomeranz Y.Discoloration of dough for oriental noodl[J].Cereal Chem,1995,72:198-205.
[19]  Parker G D,Chalmers K J,Rathjen A J,et al.Mapping loci associated with floor color in wheat(Triticum aestivum L)[J].Theor Appl Genet,1998,97:238-245.
[20]  Mares D J,Campbell A W.Mapping components of flour and noodle color in Australian wheat[J].Australian Journal of Agricultural Research,2001,52:1297-1309.
[21]  张立平,阎俊,夏先春,等.普通小麦籽粒黄色素含量的QTL 分析[J].作物学报[J],2006,32:41-45.
[22]  Kuchel H,Langridge P,Mosionek L,et al.The genetic control of milling yield,dough rheology and baking quality of wheat[J].Theor Appl Genet,2006,112:1487-1495.
[23]  Elouafi I,Nachit M M,Martin L M.Identification of a microsatellite on chromosome 7B showing a strong linkage with yellow pigment in durum wheat (Triticum turgidum L.var.durum)[J].Hereditas,2001,135:255-261.
[24]  Bagge M,Xia X C,Lübberstedt T.Functional markers in wheat[J].Current Opinion in Plant Biology,2007,10:211-216.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133