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甜瓜品种河套蜜瓜ACC氧化酶基因1(ACO1)全长cDNA的克隆及序列分析

DOI: 10.7668/hbnxb.2010.04.013, PP. 61-63

Keywords: 甜瓜,ACC氧化酶基因1,cDNA

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Abstract:

根据GenBank上登录的甜瓜ACC氧化酶基因1(Cm-ACO1)序列,设计特异性引物,应用RT-PCR从甜瓜品种河套蜜瓜成熟果实中克隆得到了ACO1的全长cDNA,共105bp,并对其进行了序列分析,结果表明,所克隆的cDNA与已报道的甜瓜品种Cantaloupcharentais的ACO1cDNA序列完全一致.该基因的克隆为进一步研究ACC氧化酶基因在甜瓜中的表达特性以及功能奠定了基础.

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