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抗草甘膦基因表达载体的构建及对玉米的遗传转化

DOI: 10.7668/hbnxb.2010.04.009, PP. 44-48

Keywords: 草甘膦,Epsps,表达载体,玉米,遗传转化

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Abstract:

以pCambia3301载体为基础,以玉米Ubiquitin(Ubi)启动子、拟南芥叶绿素转运肽(Chloroplasttransitpeptide,Ctp)基因、抗草甘膦Epsps基因为元件,构建了玉米高效双元表达载体,通过冻融法将重组质粒导入根癌农杆菌LBA4404,并通过农杆菌介导法转化玉米幼胚,获得了抗草甘膦无抗生素标记的转基因玉米植株.经田间初步检测转化玉米植株具有一定的草甘膦抗性.

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