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小麦高分子量麦谷蛋白亚基基因1By8的分子检测和应用

DOI: 10.7668/hbnxb.2010.04.007, PP. 35-39

Keywords: 小麦,高分子量麦谷蛋白,1By8亚基,分子标记检测,应用

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Abstract:

小麦的加工品质与小麦高低分子量麦谷蛋白的组成和含量密切相关,特别是Glu-1位点编码的高分子量麦谷蛋白亚基(HMW-GS)的组成和含量.为此,试验利用1By8亚基标记对黄淮麦区目前生产利用或曾经利用过的小麦品种及部分育种资源共10份进行了检测,其中有43份材料扩增出27bp特异片段,表明具有1By8亚基;107份材料未扩增出27bp片段,表明不具有1By8亚基;1By8亚基的出现频率为28.6%.所用材料的鉴定结果与SDS-PAGE电泳的结果基本一致,表明利用特异性PCR标记鉴定1By8亚基是可靠的.与SDS-PAGE相比,PCR标记更加快速、简便和准确.

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