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西马特罗人工抗原的合成及鼠源多克隆抗血清的制备

DOI: 10.7668/hbnxb.2010.04.021, PP. 97-101

Keywords: 西马特罗,人工抗原,合成,多抗血情

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Abstract:

拟合成并鉴定西马特罗(Cimaterol,CIM)人工抗原,通过动物免疫生产亲和力高、特异性好的鼠源CIM多克隆抗血清.通过重氮化方法将西马特罗偶联于载体蛋白BSA和OVA上,分别合成免疫抗原BSA-CIM和包被抗原OVA-CIM,并采用紫外分光光度法和SDS-PAGE进行了鉴定.结果表明,半抗原CIM和载体蛋白偶联成功.动物免疫试验结果显示,6只小鼠效价均达1×10-3以上,且4号小鼠多抗血清抗CIM的IC0为86.9ng/mL,表明成功获得了高效价、特异性好、亲和力高的的鼠源抗CIM多抗血清.

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