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H5N1亚型禽流感病毒核衣壳蛋白(NP)基因的进化及原核表达

DOI: 10.7668/hbnxb.2010.03.005, PP. 19-22

Keywords: 禽流感病毒,H5亚型,核衣壳蛋白(NP),原核表达

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Abstract:

参考GenBank上H5N1亚型禽流感病毒(AIV)的核衣壳蛋白(NP)基因序列设计引物,PCR扩增NP基因,对其进行序列分析并构建分子进化树。将克隆的NP基因插入原核表达载体pET32a,在大肠杆菌中进行诱导表达,对诱导表达的NP重组蛋白进行纯化、Western-Blot鉴定及免疫原性分析。结果表明,克隆的NP基因与GenBank上发表的另外2个河南AIV毒株亲缘关系较近,诱导表达的NP重组蛋白主要以可溶性形式存在,分子量约为70kDa,并且能够与H5亚型AIV阳性血清发生特异性反应,具有良好的抗原性。

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