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水分亏缺条件下豌豆肌动蛋白异型体PEAcII基因的表达

DOI: 10.7668/hbnxb.2010.03.001, PP. 1-4

Keywords: 豌豆,幼苗,肌动蛋白异型体,半定量RT-PCR

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Abstract:

以培养3~4d的豌豆幼苗为试验材料,以18SrRNA基因为内参基因,豌豆幼肌动蛋白异型体PEAcII基因为检测基因,在PEG6000模拟水分亏缺条件下,采用半定量逆转录聚合酶链式反应(RT-PCR)体系检测肌动蛋白异型体PEAcII基因在豌豆幼苗中的表达情况。在正常供水条件下,PEAcII在处理的第8h表达量最高,水分胁迫下,PEA-cII基因在处理的0.5h表达量下降,在处理的1h时表达量最高,而后下降。无论是正常供水还是水分胁迫条件下,PEAcII基因的表达都是一个动态变化的过程。试验结果表明,肌动蛋白异型体PEAcII表达量的高低可能与豌豆幼苗适应水分亏缺的生理过程有关,这对于研究豌豆幼苗抗旱性机理以及检测豌豆幼苗的抗旱性强弱具有重要意义。

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