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利用精子介导法建立hHT/hDAF双基因转移小鼠模型

DOI: 10.7668/hbnxb.2010.03.010, PP. 43-46

Keywords: 精子介导法,双基因,hHT,hDAF,转基因,小鼠

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Abstract:

为探讨超急排斥反应(Hyperacuterejection,HAR)及延迟性异种排斥反应(Delayedxenograftrejection,DXR)的分子调控机制,采用优化的精子介导法(Sperm-mediatedgenetransfer,SMGT)对建立hHT/hDAF双基因转移小鼠模型进行了研究。结果表明,应用树枝状聚合物(Dendrimers)优化SMGT后,2细胞胚GFP+RFP的阳性率达到4.51%,同对照组相比差异极显著(P<0.01)。选取标记基因GFP+RFP双阳性的胚胎进行移植后获得36只仔鼠,PCR及SouthernBlot检测结果表明,17只小鼠PCR结果呈hHT+hDAF双阳性,其中5只小鼠SouthernBlot结果呈hHT+hDAF双阳性。上述结果表明,成功建立了hHT/hDAF双基因转移小鼠模型。

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