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农杆菌介导的番茄Micro-Tom遗传转化体系的建立

DOI: 10.7668/hbnxb.2010.02.021, PP. 112-115

Keywords: Micro-Tom番茄,模式植物,根癌农杆菌,遗传转化

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Abstract:

Micro-Tom番茄植株矮小,生长密度高,生命周期短,转化效率高,成为功能基因组学研究的新型模式植物。对影响Micro-Tom遗传转化频率的共培养时间、AS的添加、工程菌液浓度和抑制农杆菌所用抗生素种类进行了分析,建立了Micro-Tom稳定高效的遗传转化体系。以bar基因设计引物对转化群体进行PCR检测,阳性率为72.3%,平均插入位点数为1.8个。遗传体系的建立为Micro-Tom在植物生物学研究中提供理论基础。

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