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Galectin-13基因在仔猪断乳前、后胃肠道组织表达差异性分析

DOI: 10.7668/hbnxb.2010.02.011, PP. 56-59

Keywords: ,Galectin-13基因,组织分布,实时荧光定量PCR,表达差异

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Abstract:

从仔猪的十二指肠组织中克隆Galectin-13基因,研究Galectin-13在仔猪不同组织中的分布情况,并采用实时荧光定量PCR检测Galectin-13基因在断乳前、后仔猪胃肠道组织中的表达差异情况。结果表明:成功克隆出了猪Galectin-13基因序列,并被GenBank收录(Accession.NM001142841)。与断乳前相比,断乳后Galectin-13基因的mR-NA在十二指肠、回肠组织中的表达量显著上调(P<0.05),而在胃、空肠、盲肠和结肠中的表达量与对照组相比差异不显著(P>0.05)。

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