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朝鲜碱茅甜菜碱醛脱氢酶cDNA5′末端序列的克隆及植物表达载体的构建

DOI: 10.7668/hbnxb.2010.02.006, PP. 30-34

Keywords: 朝鲜碱茅,甜菜碱醛脱氢酶,5&prime,RACE,高GC,表达载体

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Abstract:

采用改良的RT-PCR及5′RACE法,成功地克隆了朝鲜碱茅甜菜碱醛脱氢酶(BADH)基因cDNA5′端序列,与已知序列拼接,获得了BADHcDNA全长1781bp,包含一个完整的开放阅读框(ORF),编码506个氨基酸。其含有醛脱氢酶高度保守序列VT/SLELGGKSP,及其后29位与酶功能有关的Cys。多序列比对和系统进化分析表明,朝鲜碱茅BADH与大麦BBD1同源性最高,并构建了PBI121-BADH植物表达载体。

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