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冬瓜枯萎病菌核糖体rDNAITS区的克隆与序列分析

DOI: 10.7668/hbnxb.2010.01.015, PP. 80-83

Keywords: 冬瓜,冬瓜枯萎病菌,ITS

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Abstract:

采用镰刀菌核糖体基因转录间隔区(ITS)通用引物,PCR扩增冬瓜枯萎病菌核糖体基因ITS区,并对产物进行克隆和序列分析;利用Mega.1软件对序列及GeneBank中以葫芦科为寄主的镰刀菌不同专化型ITS序列进行聚类.冬瓜枯萎病菌ITS全长1063bp,其中包括18SrDNA一部分序列,5.8SrDNA,ITS1和ITS2全部序列及28SrDNA部分序列.聚类结果将15个菌株ITS序列划分为2个类群,类群I包括个菌株,分别为2个西瓜枯萎病菌株和2个甜瓜枯萎病菌株;类群II包括11个菌株,其中冬瓜枯萎病菌株就在该类群中,其余为甜瓜枯萎病菌株5个、西瓜枯萎病菌株3个、黄瓜枯萎病菌株、丝瓜枯萎病菌株和葫芦枯萎病菌株各1个.

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