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灵芝多糖对黄颡鱼免疫细胞活性的影响

DOI: 10.7668/hbnxb.2011.03.038, PP. 195-198

Keywords: 黄颡鱼,灵芝多糖,免疫细胞活性,呼吸爆发,细胞增殖

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Abstract:

选取50尾黄颡鱼(Pelteobagrusfulvidraco),连续投喂含不同水平灵芝多糖(300,600,900,1200,1500mg/kg)的配合饲料8周.而后采用Percoll密度梯度离心等技术,对黄颡鱼头肾巨噬细胞与外周血白细胞进行分离纯化,使用NBT还原法、Griesse试剂显色法与MTT法测定头肾巨噬细胞呼吸爆发活性与外周血白细胞的增殖能力,探讨灵芝多糖对黄颡鱼免疫细胞活性的影响.结果表明,灵芝多糖各水平组均能显著提高黄颡鱼头肾巨噬细胞的氧呼吸爆发活性和外周血白细胞的增殖能力(P<0.01);投喂灵芝多糖饲料后,各组头肾巨噬细胞氮呼吸爆发活性虽有提高,但只有1200~1500mg/kg水平组为极显著提高(P<0.01).灵芝多糖可以有效提高黄颡鱼免疫细胞的活性.

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