全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

氨苄青霉素单克隆抗体及其与青霉素类抗生素交叉反应性

DOI: 10.7668/hbnxb.2011.03.013, PP. 60-63

Keywords: 氨苄青霉素,单克隆抗体,交叉反应

Full-Text   Cite this paper   Add to My Lib

Abstract:

以戊二醛法将氨苄青霉素(Ampicillin,Amp)偶联于牛血清白蛋白(BSA)和卵清白蛋白(OVA),合成免疫抗原BSA-Amp和检测抗原0VA-Amp,并进行了鉴定;用BSA-Amp免疫BALB/c小鼠,应用杂交瘤技术建立了株分泌抗Amp单克隆抗体的杂交瘤细胞株.ELISA和竞争ELISA结果显示,单克隆抗体1G7、2A11、2D10和3F6的腹水抗体效价分别为1:2.56×107、1:1.28×107、1:5.12×106和1:5.12×106,Amp的半数抑制浓度(IC50)分别为10.22,3.58,.03,.95ng/mL.Amp单克隆抗体与羧苄青霉素(1G7、2A11、2D10和3F6)、青霉素G(1G7和3F6)、阿莫西林(1G7)等青霉素类抗生素存在显著的交叉反应,而与双氯霉素和邻氯霉素未见明显交叉反应.

References

[1]  http://www.chemicalbook.com/ProductChemicalPropertiesCB2247346_EN.htm.
[2]  Dewdney J M,Maes L,Raynaud J P,et al.Risk assessment of antibiotic residues of beta-lactams and macrolides in food products with regard to their immuno-allergic potential[J].Food Chem Toxicol,1991,29(7):477-483.
[3]  Council Regulation (EEC) No.2377/90 of 26 June 1990.Laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin[S].Off J Eur Commun,1990,L 224:34-35.
[4]  农业部畜牧兽医局.农业部发布动物性食品中兽药最高残留限量(续)[J].中国兽药杂志,2003,37(3):5-11.
[5]  Linage B,Gonzalo C,Carriedo J A,et al.Performance of blue-yellow screening test for antimicrobial detection in ovine milk[J].J Dairy Sci,2007,90(12):5374.
[6]  农业部畜牧兽医局.农业部发布动物性食品中兽药残留检测方法[J].中国兽药杂志,2003,37(3):2.
[7]  LuoW,Ang C Y W,Thompson Jr H C.Rapid method for the determination of ampicillin residues in animal muscle tissues by high-performance liquid chromatography with fluorescence detection[J].J Chromatogr B Biomed Sci Appli,1997,694(2):401-407.
[8]  Anderson K L,Moats W A,Rushing J E,et al.Ampicillin and amoxicillin residue detection in milk,using microbial receptor assay (Charm Ⅱ) and liquid chromatography methods,after extra-label administration of the drugs to lactating cows[J].Am J Vet Res,1996,57(1):73-78.
[9]  Tajiek M A,Shohreh B.Detection of antibiotics residue in chicken meat using TLC[J].Inter J Poult Sci,2006,5(7):611-612.
[10]  Cliquet P,Cox E,Van Dorpe C,et al.Generation of classselective monoclonal antibodies against the penicillin group[J].J Agric Food Chem,2001,49(7):3349-3355.
[11]  HeddyZola.单克隆抗体技术手册[M].周宗安,方元,房德兴,等译.南京:南京大学出版社,1991:31-77.
[12]  詹爱军,王新卫,陈枝楠,等.抗H9N2 AIV HA单克隆抗体的制备及其在胶体金层析检测中的初步应用[J].河南农业科学,2008(9):123-126.
[13]  赵银丽,滕蔓,邓瑞广,等.恩诺沙星单克隆抗体杂交瘤细胞株的建立及其免疫学特性[J].河南农业科学,2008(10):121-124.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133