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类猪圆环病毒因子P1感染对猪肺泡巨噬细胞外源性抗原加工递呈相关分子和共刺激分子的影响

DOI: 10.7668/hbnxb.2011.03.007, PP. 32-36

Keywords: 类猪圆环病毒因子,肺泡巨噬细胞,抗原加工和递呈相关分子,共刺激分子,实时定量PCR

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Abstract:

采用实时定量PCR技术对类猪圆环病毒因子P1感染猪不同时相猪肺泡巨噬细胞中外源性抗原加工递星相关分子SLA-DR、SLA-DM和CD74及共刺激分子CD40、CD80和CD86mRNA的表达进行了检测.结果表明,病毒感染后3d,上述分子的mRNA表达皆低于对照组相应分子的mRNA表达.其中,CD80mRNA的表达呈下调趋势(P<0.1),CD40、CD74和SLA-DR的mRNA的表达显著下调(P<0.0);感染后8d的SLA-DR和SLA-DM以及感染后17dSLA-DM的mRNA的表达有下调趋势(P<0.1);感染后24d的CD86mRNA的表达呈上调趋势(P<0.1);感染后40d的CD74mRNA的表达显著下调(P<0.0).结果揭示,类猪圆环病毒因子P1感染对猪肺泡巨噬细胞的外源性抗原加工和递呈功能有显著影响,导致其外源性抗原递呈功能下降及紊乱,进而使机体的体液免疫应答功能受到影响.

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