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山羊体外成熟卵母细胞去核时间及方法对去核效率的影响

DOI: 10.7668/hbnxb.2011.02.020, PP. 90-93

Keywords: 山羊,卵母细胞,去核

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Abstract:

在山羊卵母细胞体外成熟的不同时间,进行荧光(Hochest3332)染色,一是确定极体和细胞核染色体的位置关系,二是分别采用盲吸法和荧光指导法去核,观察去核率.结果表明:在体外成熟培养18,20,22,2h,A类卵母细胞所占比例分别为72.7%,3.7%,27.5%,16.7%,B类卵母细胞分别为9.1%,31.3%,7.8%,55.6%,C类卵母细胞分别为18.2%,25%,2.6%,27.7%;在体外成熟培养的18,20,22,2h去核,盲吸法的去核率分别为69.1%,58.2%,50.7%,5.8%,而荧光指导法去核率均为100%.

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