Khera A,Singh R,Shakila H,et al.Elicitation of efficient,protective immune responses by using DNA vaccines against tuberculosis[J].Vaccine,2005,23 (48 -49):5655-5665.
[7]
Brodin P,Rosenkrands I,Andersen P,et al.ESAT-6 proteins:protective antigens and virulence factors[J].Trends in Microbiology,2004,12(11):500-508.
[8]
Kinhikar,Arvind G,Verma,et al.Potential role for ESAT6in dissemination of M.tuberculosis viahuman lung epithelial cella[J].Molecular Molecular Microbiology,2010,75(1):92-106.
Steven C,Derrick,Sheldon L,et al.The ESAT6 protein of Mycobacterium tuberculosis induces apoptosis of macrophagesby activatingcaspase expression[J].Cellular Microbiology,2007,9 (6); 1547-1555.
[11]
Chee-Mun Fang,Zainul F,Zainuddin.Cloning,expression,and puri Wcation of recombinant protein from a single synthetic multivalent construct of Mycobacterium tuberculosis[J].Protein Expression and Puri Wcation,2006,47 ;341-347.
[12]
Shi Changhong,Zhang Hai,Wang Limei,et al.Therapeutic effcacy of a tuberculosis DNA vaccineencoding heat shockprotein 65 of Mycobacterium tuberculosis and the human interleukin 2 fusion gene[J].Tuberculosis,2009,89:54-61.
[13]
Pollock J M,Andersen P.The potential of the ESAT 6antigen secreted by virulent mycobacteria for specific diagnosis of tubereulosis[J].Infect Dis,1997,175:1251-1254.
[14]
Brian J,Rogerson,Yu-Jin Jung,et al.Expression levels of Mycobacterium tuberculosis antigen-encoding genes versus production levels of antigen-specific T cells during stationary level lung infection in mice[J].Immunology,2006,118 (5):195-201.
[15]
Lindsay J Hall,Simon Clarea,Derek Pickarda,et al.Characterisation of a live Salmonella vaccine stably expressing the Mycobacterium tuberculosis Ag85B ESAT6fusion protein[J].Vaccine,2009,27 (49):6894 -6904.
[16]
Young M D,Schneider D L,Zuckerman A J,et al.Adult hepatitis Bvaccination using a novel triple antigen recombinant vaccine[J].Hepatology,2001,34:372-376.
[17]
Chi S W,Kim D H,Lee S H,et al.Pre-structured motifs in the natively unstructured preSl surface antigen of hepatitis B virus[J].Protein Science,2007,16 (10):2108 -2117.
[18]
Park J H,Cho E W,Lee Y J,et al.Determination of the protective effects of neutralizing anti-hepatitis B virus (HBV) immunoglobulins by epitope mapping with recombinant HBV surface-antigen proteins[J].Microbiol lmmuno1,2000,44(8):703-710.
[19]
Maeng C Y,Ryu C J,Gpipon P,et al.Fine mapping of virus-neutralizing epitopes on hepatitis B virus PreS1[J].Virology,2000,270(1):9-16.
[20]
Park J H,Lee M K,Kim H S,et al.Targeted destruction of the polymerized human serum albumin binding site within the preS2 region of the HBV surface antigen while retaining full immunogenicity for this epitope[J].Viral Hepat,2003,10 (1):70-79.
Mei C,Wassom J,Widholm J M.Expression specificity of the globulin-1 promoter driven transgene(chitinase) in maize seed tissues[J].Maydica,2004,49 (4):255 -265.
