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谷子弯孢病菌Clga-1基因克隆及特征分析

DOI: 10.7668/hbnxb.2011.02.009, PP. 39-42

Keywords: 谷子弯孢病菌,异三聚体G蛋白,RACE,序列分析

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Abstract:

谷子弯孢病菌(Cochlioboluslunatus)引起的谷子叶斑病是谷子生产上的重要病害之一,常造成严重经济损失.本研究利用候选基因法和RACE技术克隆了谷子弯孢病菌中的1个Ga基因,命名为Clga-1,在GeneBank登陆号为HQ699081.Clga-1基因开放阅读框为l062bp,编码353个氨基酸多肽,该开放阅读框被3个大小分别为57,9,59bp的内含子隔开.聚类分析结果表明,谷子弯孢病菌Clga-1基因与其他植物病原真菌Ga基因的同源性很高,Southern杂交结果表明该基因以单拷贝形式存在.Clga-1基因具有其他病原真菌Ga基因具有的保守结构,如在氮端有豆蔻酰化位点,碳端具有ADP核糖基化位点.谷子弯孢病菌Clga-1基因的克隆,为进一步研究Clga-1基因在该菌致病过程中的功能奠定基础.

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