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猪Reg3基因的克隆与序列分析

DOI: 10.7668/hbnxb.2011.02.012, PP. 55-57

Keywords: ,Reg基因,克隆,序列分析

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Abstract:

在猪的肠道组织中克隆Reg基因,提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化E.coliJM109,检测阳性克隆、测序并进行序列分析.结果表明:克隆的猪Reg基因与人、绵羊、马的同源性分别为8.7%,82.%,82.2%;克隆了猪Reg全长基因并注册GenBank注册号为Accession.FJ51494.

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