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具有荧光标签的猪瘟病毒Erns蛋白在杆状病毒表达系统中的表达

DOI: 10.7668/hbnxb.2011.01.006, PP. 26-30

Keywords: 猪瘟病毒,Erns,绿色荧光蛋白,Bac-to-Bac杆状病毒表达系统

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Abstract:

为了获得具有标记的猪瘟病毒Erns蛋白,通过基因工程操作,使猪瘟病毒Erns基因与绿色荧光蛋白基因(GFP)融合,用Bac-to-Bac杆状病毒表达系统在昆虫细胞中高效表达了ErnsGFP融合蛋白,经WesternBlot和荧光显微镜观察证实,表达产物分子量正确,且发出易于检测的绿色荧光,具有标记的Erns蛋白的获得为进一步研究Erns蛋白结构和功能奠定了基础。

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