全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

表皮生长因子对牛输卵管上皮细胞生长、增殖和凋亡的影响

DOI: 10.3969/j.issn.1000-7091.2012.z1.080, PP. 410-414

Keywords: 牛输卵管上皮细胞,表皮生长因子(EGF),细胞增殖,细胞周期,细胞凋亡

Full-Text   Cite this paper   Add to My Lib

Abstract:

研究不同浓度的表皮生长因子对牛输卵管上皮细胞生长、增殖和凋亡的影响,目的是建立高质量的牛体外胚胎共培养细胞系。牛输卵管上皮细胞体外培养技术结合血球计数法检测细胞增殖,并利用流式细胞仪进行细胞周期和凋亡的检测,通过Lysis软件对数据进行分析。结果表明,EGF在0~50ng/mL浓度范围内,其促增殖、抑制凋亡作用随浓度的增加而增大,呈剂量依赖性;当浓度持续增高时,其促增殖、抑制凋亡能力降低;此外,S期细胞数量明显增加,G2-M期细胞数量有下降趋势。表皮生长因子在一定浓度范围内具有促进牛输卵管上皮细胞增殖和抑制其凋亡的作用,并使其细胞周期发生变化。

References

[1]  李 勇 , 窦忠英, 杨春荣, 等.体外生产胚胎 技术[J].中国生物工程杂志, 2003, 23: 56 - 61 .
[2]  叶 荣, 陈学进, 杨利国, 等.昆明小鼠早期胚胎体外发育阻滞原因分析[J].中国兽医学报, 2004, 24: 402 -404.
[3]  Carpenter G.The regulation of cell proliferation advance in the biology and mechanism of action of epidermalgrowth factor[J].J Invest Dematol, 1987, 71 ( 5 ) : 283 -288.
[4]  Carpenter G.Epidermal growth factor[J].Annu Rev Biochem, 1979, 48(1 ) : 193 - 216.
[5]  叶新红, 徐国江, 严敬明 .输卵管上皮细胞共培养技术对受精卵体外发育的影响及临床应用价值[J].生殖与避孕, 2001 , 21 : 183 - 187.
[6]  谭秀文, 谭景和.胚胎体外共培养: 影响因素及作用机理[J].生物工程学报, 2003, 19: 502 - 505.
[7]  Datta S R, Brunet A, Greenberg M E.Cellular survivala: a play in three Akts[J].Genes Dev, 1999, 13 ( 22) :2905 - 2927.
[8]  Wetzels A M, Bastiaans B A, Hendriks J C, et al.Theeffects of co-culture with human fibroblasts on humanembryo development in vitro and implantation[J].HumReprod, 1998, 13: 1325 - 1330.
[9]  Cohen S.Isolation of a mouse submaxilary gland proteinaccelerating incisor eruption and eyelid opening in thenew-born animal[J].J Biol Chem, 1962, 237(5) : 1555 -1557.
[10]  Real F X, Retting W J, Chesa P G, et al.Expression of epidermal growth factor receptor in human cultured cells andtissues: relationship to cell lineage and stage of differentiation.Cancer Res[J].1986, 46(9) : 4726 - 4731 .
[11]  Goldberg J M, Khalifa E A M, Frieman C I, et al.Improvement of in vitro fertilization and early embryo development in mice by co-culture with human fallopiantube epithelium[J].Am J Obstect Gynecol, 1991 , 165(12) : 1802 - 1805.
[12]  Sirisathien S, Hemandez-Fonseca H J, Brackett B G.Influence of epidermal growth factor and insulin-likegrowth factor on bovine blastocyst development in vitro [J].Anim Reprod Sci, 2003, 77(1 ) : 21 - 32.
[13]  刘以训 .胚胎的宫内和异位植入[J].中国科学 C 辑,2004, 34(2) : 105 - 112.
[14]  Goffin F, Munaut C, Malassine A, et al.Evidence of aimited contribution of feto-maternal interactions to trophoblast differentiation along the invasive pathway[J].Tissue Antigens, 2003, 62(2) : 104 - 116.
[15]  Liang R F.Effect of transforming growth factor-β and epidermal growth factor on clonal rat pulp cells[J].ArchOral Biol, 1990, 35(1 ) : 7 - 11 .
[16]  Yoneda T, Pratt R.Mesenchyal cells from the human embryonic palate are highly responsive to epidermal growthfactor[J].Science, 1981 , 213(7) : 563 - 565.
[17]  耿慧霞, 王 来, 王 强.流式细胞仪在生物学中的应用[J].生物学杂志, 2005, 22(4) : 44 - 51 .
[18]  林珏龙.流式细胞仪对凋亡细胞作用的研究[J].现代诊断与治疗, 2002, 13(4) : 226 - 229.
[19]  Lenin M, Nandini Ghosh-Choudhury, Balachandar A, etal.EGF stimulates mesangial cell mitogenesis via P13-kinase-mediated MAPK-dependent and AKT kinase-independent manner involvement of c-fos and P27 kp1[J].Am J Physiol Renal Physiol, 2005, 289(1 ) : 72 - 82.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133