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大白菜开花相关基因CO的BAC克隆筛选及分析

DOI: 10.3969/j.issn.1000-7091.2012.06.005, PP. 20-23

Keywords: 大白菜,开花相关基因,CO基因,BAC克隆

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Abstract:

根据已经公布的大白菜CO基因(Bra008669)序列设计特异引物,采用三维PCR法筛选大白菜BAC文库,获得了3个含有CO的BAC克隆。对筛选到的3个BAC克隆进行PCR扩增,将克隆测序结果与大白菜CO基因进行序列比对,结果表明,相似性达到99.73%,且N端有保守B-BOX结构,C端有保守的CCT结构域,证明所筛选的3个BAC克隆是含有大白菜CO基因的克隆。

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