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寿光地区感病辣椒TYLCV的分子鉴定和病毒DNA-A序列分析

DOI: 10.3969/j.issn.1000-7091.2012.03.009, PP. 46-49

Keywords: 辣椒:TYLCV,分子鉴定,DNA-A

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Abstract:

由于TYLCV的宿主广泛,本试验对山东寿光地区的感病毒病辣椒进行了TYLCV感染的分子鉴定,发现寿光地区的辣椒感染了TYLCV病毒,且感染率较高。通过对病毒基因组DNA-A序列的克隆和序列分析发现,寿光感病辣椒的TYLCV序列与临沂和德州的TYLCV亲缘关系最近,并对具体突变点进行了分析。进化树分析显示,克隆得到的辣椒病毒与墨西哥的TYLCV病毒同源性较高,而与危害辣椒较严重的TYLCSV的遗传距离较远。

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