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谷子弯孢病菌Clmk-1基因克隆和初步分析

DOI: 10.3969/j.issn.1000-7091.2012.03.008, PP. 41-45

Keywords: 谷子弯孢病菌,MAPK基因,基因克隆,U0126

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Abstract:

谷子弯孢病菌(Cochlioboluslunatus)为谷子上重要的叶部病害,发生严重时会影响谷子的产量及品质。为了解MAPK级联途径在谷子弯孢病菌发育及致病性中的作用,根据已知植物病原真菌MAPK基因的保守结构域设计简并引物,通过PCR扩增法获得了谷子弯孢病菌MAPK基因的同源片段,并利用RACE技术获得了基因的全长cDNA序列,该基因命名为Clmk-1。Clmk-1基因开放阅读框为1065bp,编码354个氨基酸,并且含有MAPK基因的保守的TGY和CD基序。聚类分析结果表明,谷子弯孢病菌Clmk-1基因与其他植物病原真菌Hog1类似MAPK基因的同源性很高。利用Promoterscan软件发现,Clmk-1基因启动子区含有热激转录因子(HSF)、ADR转录因子和GCR转录因子的结合元件。MAPK途径特异性抑制剂U0126抑制谷子弯孢的孢子萌发及芽管发育。研究初步表明,Clmk-1基因为Hog1类似MAPK基因,可能与渗透胁迫相关,并且MAPK级联途径参与病菌孢子萌发及芽管形态建成。克隆的Clmk-1基因为进一步研究谷子弯孢病菌MAPK基因的功能奠定基础。

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