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苹果树腐烂病菌产生细胞壁降解酶的种类及其活性分析

DOI: 10.3969/j.issn.1000-7091.2012.02.039, PP. 207-212

Keywords: 苹果树腐烂病菌,细胞壁降解酶,致病作用,木聚糖酶

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Abstract:

通过对酶活性的分析,研究证实苹果树腐烂病菌在活体外和寄主体内均能分泌一系列的细胞壁降解酶:多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、纤维素酶(Cx)、β-葡萄糖苷酶和木聚糖酶。不同碳源培养条件下,腐烂病菌产生细胞壁降解酶的活性及达到酶活性高峰的时间存在显著差异;诱导酶液对苹果愈伤组织具有明显的浸解作用,其中木聚糖为碳源产生的细胞壁降解酶,破坏能力最强。此外,研究发现,腐烂病菌在寄主体内产生细胞壁降解酶的活性变化规律不同,PMG和木聚糖酶在接种后最先被检测到活性显著升高,PMG酶活性于接种后第13天达到高峰,随后活性降低,而木聚糖酶和其他3种酶活性则随接种天数的增加活性不断增强;5种细胞壁降解酶中,Cx最大酶活性最低,而木聚糖酶活性最高,是其他酶最大活性的1.74~7.44倍。

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