全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

不同盐浓度胁迫下小麦苗期苗高和主根长的QTL分析

DOI: 10.3969/j.issn.1000-7091.2012.02.018, PP. 91-96

Keywords: 小麦,DH群体,苗高,主根长,QTL

Full-Text   Cite this paper   Add to My Lib

Abstract:

小麦苗期苗高和主根长是鉴定小麦苗期耐盐性的重要指标。利用小麦品种花培3号×豫麦57获得的DH群体168个株系,在去离子水(对照)以及50,100,200mmol/LNaCl溶液处理下,进行苗高和主根长的数量性状基因(QTL)定位分析。利用完备区间作图法,共检测到影响苗高和主根长的25个QTL,单个QTL对表型的贡献率为4.19%~23.72%。位于3D染色体区间Xgdm72-Xbarc1119上影响主根长的QTL位点具有最大的遗传效应,贡献率为23.72%;在100mmol/L和50mmol/LNaCl处理下,在2D染色体Xwmc170.2-Xgwm539区段,同时检测到影响苗高的2个QTL位点,其贡献率分别为12.59%和8.40%;在100mmol/L和200mmol/LNaCl处理下,在4D染色体Xc-fa2173-Xcfe188区段,同时检测到影响主根长的2个QTL位点,其贡献率分别为8.77%和5.70%;在对照和100mmol/LNaCl溶液处理下,在5BL染色体Xgwm213-Xswes861.2区段,同时检测到影响苗高的QTL位点,其贡献率分别为17.49%和6.28%。另外,在50mmol/LNaCl溶液处理下,4B染色体Xwmc657-Xwmc48区段还定位了1个影响苗高的QTL位点,其贡献率为12.59%;在染色体3A和染色体7D上各检测出与主根长有关的1个不同的QTL;在5A染色体Xbarc358.2-Xgwm186和Xcwem40-Xbarc358.2区间分别检测到1个影响苗高的QTL。这些主效QTL可用于苗高和主根长的分子标记辅助选择。

References

[1]  Gorham J. Partial characterization of the trait for enhanced K+/Na+discrimination in the D genome of wheat[J]. Plant Physiol,1990,180:590-597.
[2]  Gorham J. Genetic analysis and physiology of trait enhanced K+/Na+discrimination in wheat[J]. New Phytol,1997,137:109-116.
[3]  Dvorak J. Enhancenment of the salt tolerance of Triticum turgidum L. by the Knal locus transferred from the Triticum aestivum L. chromosome 4D by homoelogous recombination[J]. Theor Appl Genet,1994,87:872-877.
[4]  Dubcovsky J,Maria G S,Epstein E,et al. Mapping of the K+/Na+discrimination locus Knal in wheat[J]. Theor Apple Genet,1996,92:448-454.
[5]  索广力,黄占景,何聪芬,等. 利用 RAPD-BSA 技术筛 选小麦耐盐突变位点的分子标记[J]. 植物学报, 2001,43:598-602.
[6]  翁跃进,陈道明. 小麦耐盐基因的标记和标记的克隆
[7]  [J]. 遗传学报,2002,29:343-349.
[8]  翁跃进. 茶淀红麦耐盐基因的 RFLP 分子标记[J]. 河 北农业科学,1999,3(1):1-5.
[9]  单 雷,赵双宜,陈 芳,等. 小麦体细胞杂种山融 3 号耐盐相关 SSR 标记的筛选和初步定位[J]. 中国农 业科学,2006,9 (2):225-230.
[10]  武玉清,刘录祥,郭会君,等. 小麦苗期耐盐相关性状 的 QTL 分析[J]. 核农学报,2007,21(6):545-549.
[11]  Zhang K P,Tian J C,Zhao L,et al. Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars[J]. Genetics,2009,135:257-265.
[12]  王建康. 数量性状基因的完备区间作图方法[J]. 作 物学报,2009,35(2):239-245.
[13]  Li H,Ye G,Wang J. A modified algorithm for the improvement of composite interval mapping[J]. Genetics, 2007,175:361-374.
[14]  Li H,Ribaut J M,Li Z,Wang J. Inclusive composite interval mapping(ICIM) for digenic epistasis of quantitative traits in biparental populations[J]. Theor Appl Genet,2008,116:243-260.
[15]  Zhang L,Li H,Li Z,Wang J. Interactions between markers can be caused by the dominance effect of QTL[J]. Genetics,2008,180:1177-1190.
[16]  MeCoueh S R,Cho Y G,Yano M,et al. RePort in QTL nomenclatures[J]. Rice Genet Newsl,1997,14:11- 13.
[17]  Cao G,Zhu J,He C,Gao Y,Yan J,Wu P. Impact of epistasis and QTL environment interaction on the developmental behavior of plant height in rice(Oryza sativa L. )
[18]  [J]. Theor Appl Genet,2001,103:153-160
[19]  刘旭,史娟,张学勇,等. 小麦耐盐种质的筛选鉴定和耐盐基因的标记[J]. 植物学报,2001,43(9):948-954.
[20]  马雅琴,翁跃进. 引进春小麦品种耐盐性鉴定评价
[21]  [J]. 作物学报,2005,31(1):58-64.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133