全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

马铃薯晚疫病抗性相关基因StDIR1的克隆与表达

DOI: 10.3969/j.issn.1000-7091.2012.02.005, PP. 23-29

Keywords: Dirigent蛋白质,马铃薯晚疫病,植物抗病

Full-Text   Cite this paper   Add to My Lib

Abstract:

马铃薯是世界性粮蔬作物。致病疫霉引起的晚疫病则是马铃薯育种所面临的最严峻问题之一,因此,分离和利用马铃薯晚疫病抗性基因获得抗病新品种是马铃薯育种的重要目标。首先在连翘中发现的一类与木质素合成相关的Dirigent基因可能在植物抗病虫害过程中起重要作用。试验根据马铃薯的EST序列信息设计特异性引物,并通过RT-PCR和RACE技术获得了一条全长为729bp的Dirigent基因cDNA序列,命名为StDIR1;该cDNA编码一个包含191个氨基酸的蛋白质多肽;系统进化分析表明,该多肽是一种Dirigent-like蛋白,属于DIR-b亚群,与陆地棉Di-rigent-likeprotein1相似性高达76%,同一性为62%。利用半定量RT-PCR技术分析发现,致病疫霉、H2O2以及NO可以诱导该基因不同程度的上调表达。组织特异性分析表明,该基因在马铃薯根组织中表达量最高,而在茎、叶、花以及块茎组织中的表达量偏低。首次表明StDIR1基因与马铃薯对致病疫霉的侵染应答具有相关性。

References

[1]  Laxalt A M,Beligni M V,Lamattina L. Nitric oxide preserves the level of chlorophyll in potato leaves infectedby Phytophthora infestans[J]. Eur J Plant Pathol,1997,103:643-651.
[2]  Liang W S,Liang H G. Comparison of the effects ofH2O2and salicylic acid on alternative respiratory pathway in aged potato tuber slices[J]. Acta Bot Sin,2002,44(3):287-291.
[3]  Li G C,Jin L P,Xie K Y,et al. Clone of proteinase inhibitor gene StPI in diploid potato and its expression analysis[J]. Agric Sci China,2007,6(11):1315-1321.
[4]  Davin L B,Wang H B,Crowell A L,et al. Stereoselectivebimolecular phenoxy radical coupling by an auxiliary(dirigent) protein without an active center[J]. Science,1997,17:275-362.
[5]  Gang D R,Costa M A,Fujital M,et al. Regiochemicalcontrol of monolignol radical coupling a new paradigmfor lignin and lignan biosynthesis[J]. Chem Biol,1999,6:143-151.
[6]  陈永忠,谭晓风,Clapham D. 木质素生物合成及其基因调控研究综述[J]. 江西农业大学学报,2003,25(4):613-617.
[7]  Burlat V,Kwon M,Davin L B,et al. Dirigent proteinsand dirigent sites in lignifying tissues[J]. Phytochemistry,2001,57:883-897.
[8]  蔺占兵,马庆虎,徐 洋. 木质素的生物合成及其分子调控[J]. 自然科学进展,2003,13(5):455-461.
[9]  董海丽,井金学. 活性氧和一氧化氮在植物抗病反应中的作用[J]. 西北农林科技大学学报:自然科学版,2003,31(1):161-166.
[10]  马永毅,张志明,赵茂俊. 一氧化氮(NO)在植物抗病防卫反应中的功能[J]. 安徽农业科学,2007,35(20):6170-6174.
[11]  Song J Q,Bradeen J M,Naess S K,et al. Gene RB clonedfrom Solanum bulbocastanum confers broad spectrum resistance to potato late blight[J]. Proc Natl Acad SciUSA,2003,100(16):9128-9133.
[12]  Mizubuti E S,Fry W E. Potato late blight/ /[M]Cooke BM,Jones D G,Kaye B. The epidemiology of plant diseases. Springer Netherlands,2006,2:445-471.
[13]  杨宇红,冯兰香,谢丙炎,等. 致病疫霉有性生殖在晚疫病流行中的意义[J]. 植物保护,2003,29(5):51-54.
[14]  Ballvora A,Ercolano M R,Weiss J,et al. The R1 gene forpotato resistance to late blight(Phytophthora infestans)belongs to the leucine zipper/NBS/LRR class of plant resistance genes[J]. Plant J,2002,30(3):361-371.
[15]  郭红莲,程根武,陈捷,等. 玉米灰斑病抗性反应中酚类物质代谢作用的研究[J]. 植物病理学报,2003,33(4):342-346.
[16]  Zhu L,Zhang X,Tu L,et al. Isolation and characterization oftwo novel dirigent-like genes highly induced in cotton(Gossypium barbadense and G. hirsutum)after infection by Verticillium dahlia[J]. J Plant Pathol,2007,89(1):41-45.
[17]  Ralph S,Park J Y,Bohlmann J,et al. Dirigent proteins inconifer defense:gene discovery phylogeny and differentialwound-and insect-induced expression of a family of DIRand DIR-like genes in spruce(Picea spp.)[J]. Plant MolBiol,2006,60:21-40.
[18]  Wu R H,Wang L L,Wang Z,et al. Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica[J]. Prog Nat Sci,2009,19:347-352.
[19]  Douches D S,Kirk W W,Bertram M A,et al. Foliar andtuber assessment of late blight ( Phytophthora infestans(Mont. )de Bary)reaction in cultivated potato(Solanumtuberosum L.)[J]. Potato Res,2002,45(2):215-224.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133