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小麦杂交F1和双亲幼苗动态蛋白质组分析

DOI: 10.3969/j.issn.1000-7091.2012.02.017, PP. 85-90

Keywords: 杂种优势,动态蛋白质组,差异表达,小麦,偏双亲

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Abstract:

分别以2,3,5d叶龄的栽培小麦京411、偏硬001和杂交F1幼苗为材料,利用动态蛋白质组技术,对F1及双亲的蛋白质变化做了比较分析,结果表明,在双亲及F1中蛋白质差异表达现象被观察到,其中在5d的叶子中最为明显。如在发育2d和3d的F1叶子中,各有15个蛋白质斑点的含量产生变化,而在5d叶龄的叶子中,这种有变化的蛋白质斑点达27个,在这些变化的蛋白质中,偏双亲和低于双亲的为多数,如5d的叶子中分别占41%和44%,同时,在F1叶片中观察到有蛋白质消失和新的蛋白质诱导现象产生。这些结果表明,杂种中存在蛋白质的差异表达现象,展开这方面的研究有助于对杂种优势形成机理的理解。

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