全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

绵羊卵母细胞体外培养GhrelinmRNA的表达

DOI: 10.3969/j.issn.1000-7091.2012.02.016, PP. 81-84

Keywords: Ghrelin,卵母细胞,体外培养,实时定量RT-PCR

Full-Text   Cite this paper   Add to My Lib

Abstract:

为揭示Ghrelin在绵羊卵母细胞的表达情况,运用实时定量RT-PCR技术检测不同培养系统中绵羊卵母细胞GhrelinmRNA的相对表达量。结果表明,以M199和血清为基础培养基添加不同激素后培养的卵母细胞GhrelinmRNA相对表达量均显著上升,仅以M199为基础培养基添加不同激素后培养的卵母细胞GhrelinmRNA相对表达量均显著下降。在不同基础培养基中添加不同激素后,E2较FSH和LH有促进GhrelinmRNA相对表达量升高的趋势。绵羊卵母细胞GhrelinmRNA的表达以及在不同培养系统动力学变化,提示了这一新型分子在卵母细胞成熟过程中受激素潜在的调控作用。

References

[1]  Kojima M,Hosoda H,Date Y,et al. Ghrelin is a growthhormone-releasing acylated peptide from stomach[J]. Nature,1999,402(6762):656-660.
[2]  Furuta M,Funabashi T,Kimura F. Intracerebroventricular?administration of Ghrelin rapidly suppresses pulsatile luteinizing hormone secretion in ovariectomized rats[J]. Biochem Biophys Res Commun,2001,288(4):780-785.
[3]  Caminos J E,Tena-sempere M,Gaytan F,et al. Expression?of Ghrelin in the cyclic and pregnant rat ovary[J]. Endocrinology,2003,144(4):1594-1602.
[4]  ?Schmittgen T D,Zakrajsek B A. Effect of experimental?treatment on housekeeping gene expression:validation by?real-time,quantitative RT-PCR[J]. J Biochem Biophys?Methods,2000,46(112):69-81.
[5]  Miller D W,Harrison J L,Brown Y A,et al. Immunohistochemical evidence for an endocrine/paracrine role for Ghrelin in the reproductive tissues of sheep[J]. Reprod Biol?Endocrinol,2005,3:60.
[6]  Gaytan F,Barreiro M L,Chopin L K,et al. Immunolocalization of Ghrelin and its functional receptor,the type 1a?growth hormone secretagogue receptor,in the cyclic human ovary[J]. J Clin Endocrinol Metab,2003,88(2):?879-887.
[7]  Kawamura K,Sato N,Fukuda J,et al. Ghrelin inhibits the?development of mouse preimplantation embryos in vitro
[8]  [J]. Endocrinology,2003,144(6):2623-2633.
[9]  Crosby I M,Gandolfi F,Moor R M. Control of protein synthesis during early cleavage of sheep embryos[J]. J Reprod Fertil,1988,82(2):769-775.
[10]  杜晨光,曹贵方. 绵羊卵泡内 Ghrelin 的免疫组化定位
[11]  [J]. 华北农学报,2008,23(3):151-153.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133