全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

玉米ZmCBL2-2基因的克隆及其表达分析

DOI: 10.3969/j.issn.1000-7091.2012.02.006, PP. 30-34

Keywords: 玉米,CBL,基因克隆,表达分析

Full-Text   Cite this paper   Add to My Lib

Abstract:

为通过转基因植物等方法分析玉米ZmCBL2-2基因在耐逆性中的功能,运用RT-PCR(ReversetranscriptionPCR)方法克隆了该基因的编码区cDNA片段,并将其构建到植物表达载体p3301(pCAMBIA3301)上。同时,运用荧光实时定量PCR(Real-timequantitativePCR,RT-qPCR)技术对玉米幼苗期该基因在高盐、低温和养分缺乏等6种非生物胁迫下的表达动态进行了分析,发现其对于缺N、低K、低温、外源ABA(Abscisicacid)和水分胁迫均有显著的应答反应,而对于盐胁迫无明显的响应。

References

[1]  Reddy V S,Reddy A S N. Proteomics of calcium-signaling?components in plants[J]. Phytochemistry,2004,65:1750?-1776.
[2]  Batistic O,Kudla J. Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein?kinase network[J]. Planta,2004,219:915-924.
[3]  Kolukisaoglu U,Wein S,Blazevic D,et al. Calcium sensors and their interacting protein kinases:genomics of the?Arabidopsis and rice CBL-CIPK signaling networks[J].?Plant Physiol,2004,134:43-58.
[4]  Luan S,Kudla J,Rodriguez-Concepcion M,et al. Calmodulins and calcineurin B-like proteins:calcium sensors for?specific signal response coupling in plants[J]. Plant?Cell,2002,14(Suppl):389-400.
[5]  Mart nez-Atienza J,Jiang X,Garciadeblas B,et al. Conservation of the Salt Overly Sensitive pathway in rice[J].?Plant Physiol,2007,143:1001-1012.
[6]  Cheong Y H,Kim K N,Pandey G K,et al. CBL1,a calcium sensor that differentially regulates salt,drought,and?cold responses in Arabidopsis[J]. Plant Cell,2003,15:?1833-1845.
[7]  Cheong Y H,Pandey G K,Grant J J,et al. Two calcineurin B-like calcium sensors,interacting with protein?kinase CIPK23,regulate leaf transpiration and root potassium uptake in Arabidopsis[J]. Plant Journal,2007,52:?223-239.
[8]  Xu J,Li H D,Chen L Q,et al. A protein kinase,interacting with two calcineurin B-like proteins,regulates K+?transporter AKT1 in Arabidopsis[J]. Cell,2006,125:?1347-1360.
[9]  李利斌,刘开昌,王殿峰,等. 玉米 CBL 基因的生物信?息学分析[J]. 玉米科学,2010,18 (1):6-11.
[10]  ?Wang Maoyan,Gu Dan,Liu Tingsong,et al. Overexpression of a putative maize calcineurin B-like protein in?Arabidopsis confers salt tolerance[J]. Plant Mol Bio, 2007,65:733-746.
[11]  Zhao Jinfeng,Sun Zhenfei,Zheng Jun,et al. Cloning and?characterization of a novel CBL-interacting protein kinase from maize[J]. Plant Mol Biol,2009,69:661-?674.
[12]  高大中,林清芳,王存芳,等. 玉米 ZmCBL6 基因的克?隆及其植物表达载体构建[J]. 内蒙古农业大学学?报,2009,30 (4):153-162.
[13]  王存芳,李记园,王茅雁. 玉米 ZmCBL5-2 基因的克隆?及其转化拟南芥[J]. 内蒙古农业大学学报,2011,32?(1):1-5.
[14]  Livak K J,Schmittgen T D. Analyzing real-time PCR data by the 2- ΔΔCTmethod[J]. Nature Protocols,2001,3:?1101-1108.
[15]  Girdhar K,Pandey. Emergence of a novel calcium signaling pathway in plants:CBL-CIPK signaling network[J].?Physiol Mol Biol Plants,2008,14:1-2.
[16]  Kudla J,Xu Q,Harter K,et al. Genes for calcineurin Blike proteins in Arabidopsis are differentially regulated by?stress signals[J]. Proc Natl Acad Sci USA,1999,96:?4718-4723.
[17]  ?Zhang Hechen,Yin Weilun,Xia Xinli. Calcineurin BLike family in Populus: comparative genome analysis?and expression pattern under cold,drought and salt?stress treatment[J]. Plant Growth Regul,2008,56:129?-140.
[18]  Pandey G K,Cheong Y H,Kim K N,et al. The calcium?sensor calcineurin B-like 9 modulates Abscisic Acid sensitivity and biosynthesis in Arabidopsis [J]. Plant Cell, 2004,16:1912-1924.
[19]  Hwang Y S,Bethke P C,Cheong Y H,et al. A gibberellin-regulated calcineurin B in rice localizes to the tonoplast and is implicated in vacuole function[J]. Plant?Physiol,2005,138:1347-1358.
[20]  ?Liu J,Zhu J K. A calcium sensor homolog required for?plant salt tolerance[J]. Science,1998,280:1943-1945.
[21]  Guo Y,Halfter U,Ishitani M,et al. Molecular characterization of functional domains in the protein kinase SOS2?that is required for plant salt tolerance[J]. Plant Cell, 2001,13:1383-1399.
[22]  Guo Y,Qiu Q S,Quintero F J,et al. Transgenic evaluation?of activated mutant alleles of SOS2 reveals a critical requirement for its kinase activity and C-terminal regulatory?domain for salt tolerance in Arabidopsis thaliana [J].?Plant Cell,2004,16:435-449.
[23]  Zhu J K. Regulation of ion homeostasis under salt stress
[24]  [J]. Curr Opin Plant Bio,2003,16:441-445.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133