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水稻丙酮酸激酶基因OsPK1的过表达转基因株系的获得与鉴定

DOI: 10.3969/j.issn.1000-7091.2012.02.001, PP. 1-5

Keywords: OsPK1,过表达,丙酮酸激酶,水稻

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Abstract:

利用农杆菌介导的转化方法研究OsPK1过表达对水稻的影响。将CaMV35S启动子驱动的OsPK1的全长CDS序列导入到日本晴基因组中。选择3个过表达转基因系作为代表进行鉴定和分析。结果显示,过表达植株在株高、分蘖数、穗长和千粒重四方面接近野生型水稻,结实率略下降,但每穗饱满种子数明显增加。通过定量RT-PCR分析,发现有4个代谢酶在过表达植株嫩叶中表达存在着上调或下调。用GC-MS方法检测糖含量,显示OsPK1过表达株系中葡萄糖、果糖、半乳糖和蔗糖的含量与野生型差异不大。总之,对OsPK1过表达株系的鉴定,有助于更好了解OsPK1的功能,并且每穗饱满种子数明显增加具潜在的应用价值。

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