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河南省豫西地区农田土壤镰孢菌种群多样性分析

DOI: 10.3969/j.issn.1000-7091.2012.02.038, PP. 202-206

Keywords: 镰孢菌,种群多样性,系统发育学

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Abstract:

采用形态学和现代分子生物学方法,对河南省豫西地区农田土壤镰孢菌种群多样性进行了分析。通过鉴定,共得到了9个种的镰孢菌,分别为尖孢镰孢菌、半裸镰孢菌、轮枝镰孢菌、禾谷镰孢菌、层生镰孢菌、锐顶镰孢菌、黄色镰孢菌、木贼镰孢菌和茄腐镰孢菌。系统发育学分析结果表明:镰孢菌属真菌18SrDNA-ITS和EF-1α基因序列均具有一定的同源性和丰富的遗传多态性,其中李瑟组镰孢菌轮枝镰孢菌、层生镰孢菌、黄色镰孢菌种间18SrDNA-ITS基因序列存在着较高的同源性,不能正确地表现镰孢菌属种一级分类单元的特征;而延伸因子EF-1α基因序列却较好地表现出了供试菌株种一级的关系,说明镰孢菌延伸因子EF-1α基因序列较18SrDNA-ITS基因序列存在更丰富的种间多态性,可以较好地表现镰孢菌种一级的关系。

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