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东亚砂藓bZIP转录因子RjbZIP基因的克隆及表达分析

DOI: 10.7668/hbnxb.2015.04.003, PP. 13-20

Keywords: 东亚砂藓,bZIP转录因子,RjbZIP基因,基因克隆,表达分析

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Abstract:

为研究bZIP转录因子在苔藓植物中的生物学功能,以东亚砂藓转录组测序获得的一个与bZIP转录因子同源性较高的基因片段为基础,采用RT-PCR技术克隆得到该基因的cDNA全长序列,命名为RjbZIP。该基因cDNA全长为1477bp,包含1个1422bp的开放阅读框,编码473个氨基酸,预测蛋白分子量为5.114kDa,等电点为6.97。RjbZIP蛋白属于不稳定蛋白,无跨膜区和信号肽结构,亚细胞定位于细胞核。该蛋白含有典型的bZIP结构域,该结构域包含一个亮氨酸拉链区,一个碱性结构域和一个谷氨酰胺丰富区。实时荧光定量PCR分析显示,在快速脱水、缓慢脱水和脱水后复水处理过程中,东亚砂藓RjbZIP基因均能被诱导表达,且对脱水后复水的反应更为迅速,推测该基因参与东亚砂藓抵抗逆境胁迫的应答,为后续进一步研究其功能特征奠定了基础。

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