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Ghrelin对小鼠不同来源胚胎体外发育的影响及其受体的表达模式

DOI: 10.7668/hbnxb.2015.04.014, PP. 78-83

Keywords: Ghrelin,Ghsr-1a,小鼠,胚胎

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Abstract:

为探明Ghrelin及其受体Ghsr-1a在早期胚胎发育中的作用,以小鼠体内受精、体外受精和孤雌激活胚胎为研究对象,在其体外发育过程中添加不同浓度Ghrelin,观察胚胎发育效率;通过免疫荧光染色和荧光定量PCR,观察不同时期胚胎中Ghsr-1a的变化。结果表明,体内受精胚体外发育中添加0.1ng/mLGhrelin显著降低了囊胚率(P<0.05),添加1ng/mL的Ghrelin显著降低了小鼠体外受精胚的囊胚率(P<0.05)。在体内受精胎中,Ghsr-1a的mRNA表达水平在4细胞显著高于其他时期(P<0.05);体外受精胚中,Ghsr-1a的mRNA水平在8细胞前无明显差异,桑椹胚和囊胚显著下降(P<0.05)。在孤雌激活胚胎中,Ghsr-1a的mRNA表达水平从2细胞到8细胞显著降低,之后到囊胚期开始显著升高,其蛋白表达模式与mRNA表达模式相反。Ghsr-1a在体内受精和体外受精胚胎中的表达相似,囊胚期表达量最低。结果提示,Ghrelin能够抑制体内和体外受精胚的体外发育,不同来源胚胎Ghsr-1amRNA和蛋白表达模式均不相同。研究结果为进一步探索Ghrelin在胚胎发育中的作用机制提供了试验基础。

References

[1]  Summers M C,Bhatnagar P R,Lawitts J A,et al.Fertilization in vitro of mouse ova from inbred and outbred strains:complete preimplantation embryo development in glucose-supplemented KSOM[J].Biology of Reproduction,1995,53(2):431-437.
[2]  Quinn P,Kerin J F,Warnes G M.Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid[J].Fertility and Sterility,1985,44(4):493-498.
[3]  Popelkova M,Sirotkin A V,Bezakova A,et al.Effect of IGF-I,leptin,ghrelin and MAPK-ERK on the nuclear maturation of bovine oocytes[J].Bulletin of the Veterinary Institute in Pulawy,2006,50(2):179-181.
[4]  Dovolou E,Periquesta E,Messinis I E,et al.Daily supplementation with ghrelin improves in vitro bovine blastocysts formation rate and alters gene expression related to embryo quality[J].Theriogenology,2014,81(4):565-571.
[5]  Mckee K K,Palyha O C,Feighner S D,et al.Molecular analysis of rat pituitary and hypothalamic growth hormone secretagogue receptors[J].Molecular Endocrinology,1997,11(4):415-423.
[6]  Gualillo O,Lago F,Gómez-Reino J,et al.Ghrelin,a widespread hormone:insights into molecular and cellular regulation of its expression and mechanism of action[J].FEBS Letters,2003,552(2/3):105-109.
[7]  Horvath T L,Diano S,Sotonyi P,et al.Minireview:ghrelin and the regulation of energy balance-a hypothalamic perspective[J].Endocrinology,2001,142(10):4163-4169.
[8]  Garcia M C,Lopez M,Alvarez C V,et al.Role of ghrelin in reproduction[J].Reproduction,2007,133(3):531-540.
[9]  Harrison J L,Adam C L,Brown Y A,et al.An immunohistochemical study of the localization and developmental expression of ghrelin and its functional receptor in the ovine placenta[J].Reproductive Biology and Endocrinology,2007,5:25.
[10]  Kawamura K,Sato N,Fukuda J,et al.Ghrelin inhibits the development of mouse preimplantation embryos in vitro [J].Endocrinology,2003,144(6):2623-2633.
[11]  Tena-Sempere M.Ghrelin and reproduction:ghrelin as novel regulator of the gonadotropic axis[J].Vitamins and Hormones,2008,77:285-300.
[12]  Dupont J,Maillard V,Coyral-Castel S,et al.Ghrelin in female and male reproduction[J].International Journal of Peptides,2010:158102.
[13]  Fernández-Fernández R,Tena-Sempere M,Navarro V M,et al.Effects of ghrelin upon gonadotropin-releasing hormone and gonadotropin secretion in adult female rats: in vivo and in vitro studies[J].Neuroendocrinology,2005,82(5/6):245-255.
[14]  Fernández-Fernández R,Navarro V M,Barreiro M L,et al.Effects of chronic hyperghrelinemia on puberty onset and pregnancy outcome in the rat[J].Endocrinology,2005,146(7):3018-3025.
[15]  Li L,Ferin M,Sauer M V,et al.Serum and follicular fluid ghrelin levels negatively reflect human oocyte quality and in vitro embryo development[J].Fertility and Sterility,2011,96(5):1116-1120.
[16]  Dovolou E,Messinis I E,Periquesta E,et al.Ghrelin accelerates in vitro maturation of bovine oocytes[J].Reproduction in Domestic Animals,2014,49(4):665-672.
[17]  谢体三,农 微,张新民,等.Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响[J].中国兽医学报,2009,29(11):1478-1480.
[18]  Wang Z,Lin P,Yu S.Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos[J].Theriogenology,2013,79(4):695-701.
[19]  Zhang K,Wei H X,Zhang Y H,et al.Effects of ghrelin on in vitro development of porcine in vitro fertilized and parthenogenetic embryos[J].The Journal of Reproduction and Development,2007,53(3):647-653.
[20]  Du C,Li H,Cao G,et al.Expression of the orexigenic peptide ghrelin and the type 1a growth hormone secretagogue receptor in sheep oocytes and pre-implantation embryos produced in vitro [J].Reproduction in Domestic Animals,2010,45(1):92-98.

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