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食欲素A对绵羊卵巢颗粒细胞P-AMPK与P-ERK信号通路的影响

DOI: 10.7668/hbnxb.2015.03.024, PP. 136-139

Keywords: 食欲素A,颗粒细胞,P-AMPK,P-ERK

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Abstract:

为研究食欲素A对绵羊卵巢能量代谢及生长发育的调节作用,在体外培养黄体化颗粒细胞,用浓度为0.05,0.10μg/mL的食欲素A刺激细胞后,分别于0,2,6,12,24h提取细胞总蛋白,采用WesternBlot法对P-AMPK及P-ERK的表达量进行检测。结果显示,食欲素A刺激细胞2h,0.05,0.10μg/mL剂量组的P-AMPK表达量与对照组相比均极显著提高(P<0.01),食欲素A刺激细胞6h,0.05,0.10μg/mL剂量组P-AMPK的表达量均达到最高值(P<0.01),且0.10μg/mL剂量组表达量明显高于0.05μg/mL剂量组;食欲素A刺激细胞2h,0.05,0.10μg/mL剂量组的P-ERK表达量与对照组相比均极显著提高(P<0.01),食欲素A刺激细胞24h,0.10μg/mL剂量组的P-ERK表达量极显著高于平台期(6~12h)(P<0.01)并达到最高值。表明食欲素A通过P-AMPK和P-ERK途径参与黄体能量代谢及生长发育的调节,从而进一步参与生殖机能的调控。

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