全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

灰葡萄孢致病力增强突变体BCt98的突变基因分析

DOI: 10.7668/hbnxb.2015.03.007, PP. 37-41

Keywords: 灰葡萄孢,突变体BCt98,致病力,突变基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

为获得灰葡萄孢的致病相关基因并研究其基因功能,筛选灰葡萄孢的T-DNA插入突变体库,获得了一株致病力增强的突变体BCt98。利用PCR和SouthernBlotting技术,对突变体BCt98进行鉴定。利用TAIL-PCR技术结合生物信息学方法,确定了突变体BCt98中T-DNA插入位点位于BC1G_07014.1基因的第3个外显子上。利用RT-PCR技术,确定了突变体BCt98的突变基因为BC1G_07014.1。突变体BCt98生长速度较快,菌落颜色较浅,菌丝较为致密,不产生分生孢子和菌核,且胞壁降解酶(PMG、PG和Cx)及毒素活性较野生型明显增强。表明BC1G_07014.1基因在灰葡萄孢生长、发育和致病力调控方面发挥重要作用,且参与调控病菌的胞壁降解酶活性和毒素活性。

References

[1]  Brito N,Espino J J,González C.The endo-beta-1,4-xylanase xyn11A is required for virulence in Botrytis cinerea [J].Molecular Plant-microbe Interactions,2006,19(1):25-32.
[2]  Cui Z,Ding Z,Yang X,et al.Gene disruption and characterization of a class V chitin synthase in Botrytis cinerea [J].Canadian Journal of Microbiology,2009,55(11):1267-1274.
[3]  Levis C,Giraud T,Dutertre M,et al.Telomeric DNA of Botrytis cinerea :a useful tool for strain identification[J].FEMS Microbiology Letters,1997,157(2):267-272.
[4]  Williamson B,Tudzynski B,Tudzynski P,et al. Botrytis cinerea :the cause of grey mould disease[J].Molecular Plant Pathology,2007,8(5):561-580.
[5]  Giraud T,Fortini D,Levis C,et al.RFLP markers show genetic recombination in Botrytis fuckeliana (Botrytis cinerea) and transposable elements reveal two sympatric species [J].Molecular Biology and Evolution,1997,14:1177-1185.
[6]  Van Kan J A.Licensed to kill:the lifestyle of a necrotrophic plant pathogen[J].Trends in Plant Science,2006,11(5):247-253.
[7]  Williamson B,Tudzynski B,Tudzynski P,et al. Botrytis cinerea :the cause of grey mould disease[J].Molecular Plant Pathology,2007,8(5):561-580.
[8]  Choquer M,Fournier E,Kunz C,et al. Botrytis cinerea virulence factors:new insights into a necrotrophic and polyphageous pathogen[J].FEMS Microbiology Letters,2007,277(1):1-10.
[9]  安鑫龙,董金皋,韩建民.玉米大斑病菌的RAPD分析Ⅰ.应用CTAB法提取玉米大斑病菌DNA[J].河北农业大学学报,2001,24(1):38-41.
[10]  Mullins E D,Chen X,Romaine P,et al.Agrobacterium-Mediated transformation of Fusarium oxysporum :an efficient tool for insertional mutagenesis and gene transfer[J].Phytopathology,2001,91(2):173-180.
[11]  李培芬,赵福鑫,董丽萍,等.灰葡萄孢BcKMO在病菌生长、发育和致病过程中的功能[J].中国农业科学,2014,47(15):2971-2979.
[12]  吴洁云.灰葡萄孢角质酶,胞壁降解酶种类及其对番茄植株的致病作用[D].扬州:扬州大学,2007:26-31.
[13]  徐 扩,张金林,侯淑英,等.BK2-灰葡萄孢产生的一种有除草活性的毒素组分[J].植物保护学报,2006,33(1):111-112.
[14]  Gourgues M,Brunet-Simon A,Lebrun M H,et al.The tetraspanin BcPls1 is required for appressorium-mediated penetration of Botrytis cinerea into host plant leaves[J].Molecular Microbiology,2004,51(3):619-629.
[15]  Schoonbeek H,Del S G,De waard M A.The ABC Transporter BcatrB affects the sensitivity of Botrytis cinerea to the phytoalexin resveratrol and the fungicide fenpiclonil[J].Molecular Plant-microbe Interactions,2001,14(4):562-571.
[16]  Segmüller N,Ellendorf U,Tudzynski B,et al.BcSAK1,a stress-activated mitogen-activated protein kinase,is involved in vegetative differentiation and pathogenicity in Botrytis cinerea [J].Eukaryotic Cell,2007,6(2):211-221.
[17]  Siewers V,Viaud M,Jimenez-Teja D,et al.Functional analysis of the cytochrome P450 monooxygenase gene bcbot1 of Botrytis cinerea indicates that botrydial is a strain-specific virulence factor[J].Molecular Plant-microbe Interactions,2005,18(6):602-612.
[18]  Soulié M C,Perino C,Piffeteau A,et al. Botrytis cinerea virulence is drastically reduced after disruption of chitin synthase class Ⅲ gene (Bcchs3a)[J].Cellular Microbiology,2006,8(8):1310-1321.
[19]  Soulie M C,Piffeteau A,Choquer M,et al.Disruption of Botrytis cinerea class Ⅰ chitin synthase gene bcchs1 results in cell wall weakening and reduced virulence[J].Fungal Genetics and Biology,2003,40(1):38-46.
[20]  Valette-Collet O,Cimerman A,Reignault P,et al.Disruption of Botrytis cinerea pectin methylesterase gene bcpme1 reduces virulence on several host plants[J].Molecular Plant-microbe Interactions,2003,16(4):360-367.
[21]  Viaud M,Brunet-Simon A,Brygoo Y,et al.Cyclophilin a and calcineurin functions investigated by gene inactivation,cyclosporin a inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea [J].Molecular Microbiology,2003,50(5):1451-1465.
[22]  Viaud M,Fillinger S,Liu W,et al.A class Ⅲ histidine kinase acts as a novel virulence factor in Botrytis cinerea [J].Molecular Plant-microbe Interactions,2006,19(9):1042-1050.
[23]  Yamane Y I,Fujita J,Shimizu R I,et al.Production of cellulose-and xylan-degrading enzymes by a koji mold, Aspergillus oryzae,and their contribution to the maceration of rice endosperm cell wall[J].Journal of Bioscience and Bioengineering,2002,93(1):9-14.
[24]  李万昌,王俊伟,彭 陈,等.真菌质膜H+-ATPase的研究进展[J].江苏农业科学,2013,41(11):16-18,19.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133