全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

栽培花生品种间SSR标记多态性及特征分析

DOI: 10.7668/hbnxb.2015.02.032, PP. 183-190

Keywords: 花生,SSR,多态性,特征

Full-Text   Cite this paper   Add to My Lib

Abstract:

通过筛选栽培花生间具有多态性的SSR,分析总结多态性SSR的特征,旨在选择性利用SSR标记,推动栽培种花生遗传图谱构建、相关农艺性状的QTL定位和分子标记辅助育种进程。以秦皇岛光秧、石腰豆、印度窄叶、抗019、四粒红和黑花生6个不同植物学类型的中国栽培种花生为材料,选用不同设计类型的1523个SSR标记筛选品种间多态性SSR,并对其扩增片段长度、重复基序、重复次数等进行分析。结果表明,1523个标记中,筛选获得43对多态性SSR标记,产生123个多态性标记位点,多态性标记数占2.8%。43对多态性SSR标记中,以ARS××的多态性最高,在6个品种中多态率达6.8%。统计各种质间具多态性的SSR,其中石腰豆与四粒红间多态性最高,共筛选到56个多态性标记位点;秦皇岛光秧与印度窄叶间多态率最低,筛选到21个多态性标记位点。在123个多态性SSR标记位点中,80%多态性SSR位点片段长度为150~300bp;18.8%多态标记的重复基序为GA,其次为AG、TC,分别占12.5%,12.5%;多态标记中二核苷酸、三核苷酸重复基序的重复次数在15次以上的占60%。

References

[1]  孙大容.花生育种学[M].北京:中国农业出版社,1998:21.
[2]  Gautami B,Ravi K,Narasu M L,et al.Novel set of groundnut SSR markers for germplasm analysis and interspecific transferability[J].International Journal of Integrative Biology,2009,7(2):100-106.
[3]  Guo B Z,Pandey M K,He G H,et al.Recent advances in molecular genetic linkage maps of cultivated peanut[J].Peanut Science,2013,40:95-106.
[4]  Moretzsohn M C,Leoi L,Proite K,et al.A microsatellite-based,gene-rich linkage map for the AA genome of Arachis (Fabaceae)[J].Theoretical and Applied Genetics,2005,111(6):1060-1071.
[5]  Ravi K,Vadez V,Isobe S,et al.Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut(Arachis hypogaea L.)[J].Theor Appl Genet,2011,122:1119-1132.
[6]  Qin H D,Feng S P,Chen C,et al.An integrated genetic linkage map of cultivated peanut (Arachis hypogaea L.) constructed from two RIL populations[J].Theoretical and Applied Genetics,2012,124(4):653-664.
[7]  Zhao Y L,Prakash C S,He G H.Characterization and compilation of polymorphic simple sequence repeat (SSR) markers of peanut from public database[J].BMC Research Notes,2012,5:362.
[8]  Pandey M K,Gautami B,Jayakumar T A,et al.Highly informative genic and genomic SSR markers to facilitate molecular breeding in cultivated groundnut (Arachis hypogaea L.)[J].Plant Breeding,2012,131(1):139-147.
[9]  Boontang S,Tantisuwichwong N,Jogloy S,et al.Simple sequence repeat (SSR)-based genetic variability among peanut genotypes different in specific leaf weight and relative water content[J].African Journal of Biotechnology,2013,12(26):4053-4064.
[10]  崔顺立,刘立峰,陈焕英,等.河北省花生地方品种基于SSR标记的遗传多样性[J].中国农业科学,2009,42(9):3346-3353.
[11]  Gautami B,Foncéka D,Pandey M K,et al.An international reference consensus genetic map with 897 marker loci based on 11 mapping populations for tetraploid groundnut (Arachis hypogaea L.)[J].PLoS One,2012,7(7):1-11.
[12]  Shirasawa K,Koilkonda P,Aoki K,et al.Sin silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map incultivated peanut[J].BMC Plant Biology,2012,12:80.
[13]  Gimenes M A,Hoshino A A,Barbosa A V,et al.Characterization and transferability of microsatellite markers of the cultivated peanut(Arachis hypogaea) [J].BMC Plant Biology,2007,7:9.
[14]  Hopkins M S,Casa A M,Wang T,et al.Discovery and characterization of polymorphic simple sequence repeats (SSRs) in peanut[J].Crop Sci,1999,39:1243-1247.
[15]  Moretzsohn M C,Hopkins M S,Mitchell S E,et al.Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome[J].BMC Plant Biology,2004,4:11.
[16]  Macedo S E,Moretzsohn M C,Leal-Bertioli S C M,et al.Development and characterization of highly polymorphic long TC repeat microsatellite markers for genetic analysis of peanut[J].BMC Research Notes,2012,5:86.
[17]  Moretzsohn M C,Barbosa A V G,Alves-freitas D M T,et al.A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome[J].BMC Plant Biology,2009,9:40.
[18]  Guo B Z,Chen X P,Hong Y B,et al.Analysis of gene expression profiles in leaf tissues of cultivated peanuts and development of EST-SSR markers and gene discovery[J].Intl J Plant Genomics,2009:1-14.
[19]  Cuc L M,Mace E S,Crouch J H,et al.Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea L.)[J].BMC Plant Biology,2008,8:55.
[20]  He G H,Meng R H,Newman M,et al.Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.)[J].BMC Plant Biology,2003,3:3.
[21]  Proite K,Leal-Bertioli S C M,Bertioli D J,et al.ESTs from a wild Arachis species for gene discovery and marker development[J].BMC Plant Biology,2007,7:7.
[22]  Ferguson M E,Burow M D,Schulze S R,et al.Microsatellite identification and characterization in peanut (A.hypogaea L.)[J].Theoretical and Applied Genetics,2004,108:1064-1070.
[23]  Wang C T,Yang X D,Chen D X,et al.Isolation of simple sequence repeats from groundnut[J].Electronic Journal of Biotechnology,2007,10(3):473-480.
[24]  姜慧芳,任小平,张晓杰,等.中国花生小核心种质与ICRISAT微核心种质的SSR遗传多样性比较[J].作物学报,2010,36(7):1084-1091.
[25]  姜慧芳,任小平,张晓杰,等.中国花生小核心种质SSR遗传多样性[J].中国油料作物学报,2010,32(4):472-478.
[26]  Eujayl I,Sorrells M E,Baum M,et al.Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat[J].Theoretical and Applied Genetics,2002,104(2/3):399-407.
[27]  Gupta P K,Rustgi S,Sharma S,et al.Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat[J].Molecular Genetics and Genomics,2003,270(4):315-323.
[28]  Cordeiro G M,Casu R,Mcintyre C L,et al.Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum[J].Plant Science,2001,160(6):1115-1123.
[29]  Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109(1):122-128.
[30]  Varshney R K,Bertioli D J,Moretzsohn M C,et al.The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.) [J].Theoretical and Applied Genetics,2009,118(4):729-739.
[31]  Liang X,Chen X,Hong Y,et al.Utility of EST-derived SSR in cultivated peanut(Arachis hypogaea L.)and Arachis wild species[J].BMC Plant Biology,2009:35.
[32]  Li Y,Chen C Y,Knapp S J,et al.Characterization of simple sequence repeat (SSR) markers and genetic relationships within cultivated peanut (Arachis hypogaea L.)[J].Peanut Science,2011,38:1-10.
[33]  胡宏霞,穆国俊,侯名语,等.河北省花生地方品种基于EST-SSR的遗传多样性及性状-标记相关分析[J].植物遗传资源学报,2013,14(6):1118-1123,1129.
[34]  Mondal S,Hande P,Badigannavar A M.Identification of transposable element markers for a rust (Puccinia arachidis Speg.) resistance gene in cultivated peanut[J].Journal of Phytopathology,2014,162(7-8):548-552.
[35]  Wang Z,Weber J,Zhong G,et al.Survey of short tandem DNA repeats[J].Theoretical and Applied Genetics,1994,88:1-6.
[36]  Udupa S M,Robertson L D,Weigand F,et al.Allelic variation at (TAA)n microsatellite loci in a world collection of chickpea (Cicer arietinum L.) germplasm[J].Mol Gen Genet,1999,261:354-363.
[37]  Burstin J,Deniot G,Potier J,et al.Microsatellite polymorphism in Pisum sativum[J].Plant Breeding,2001,120(4):311-317.
[38]  Love J,Knight A,McAleer M,et al.Towards construction of a high-resolution map of the mouse genome using PCR analyzed microsatellites[J].Nucleic Acids Research,1990,18:4123-4130.
[39]  Bell C,Ecker J.Assignment of 30 microsatellite loci to the linkage map of Arabidopsis[J].Genomics,1994,19:137-144.
[40]  Yu K,Park S,Poysa V.Abundance and variation of microsatellite DNA sequences in beans (Phaseolus and Vigna)[J].Genome,1999,42:27-34.
[41]  Cuc L M,Mace E S,Crouch J H,et al.Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea L.)[J].BMC Plant Biology,2008,8:55.
[42]  Hong Y B,Chen X P,Liang X Q,et al.A SSR-based composite genetic linkage map for the clutivated peanut(Arachis hypogaea L.)genome[J].BMC Plant Biology,2010,10:17.doi:10.1186/1471-2229-10-17.
[43]  洪彦彬,梁炫强,陈小平,等.花生栽培种SSR遗传图谱的构建[J].作物学报,2009,35(3):395-402.
[44]  张新友,韩锁义,徐 静,等.花生主要品质性状的QTLs定位分析[J].中国油料作物学报,2012,34(3):311-315.
[45]  姜慧芳,任小平,陈玉宁,等.中国花生地方品种与育成品种的遗传多样性[J].西北植物学报,2011,31(8):1551-1559.
[46]  任小平,张晓杰,廖伯寿,等.ICRISAT花生微核心种质资源SSR标记遗传多样性分析[J].中国农业科学,2010,43(14):2848-2858.
[47]  Cho Y G,Ishii T,Temnykh S,et al.Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.) [J].Theoretical and Applied Genetics,2000,100(5):713-722.
[48]  Weber J L.Informativeness of human (dC-dA)n (dG-dT)n polymorphisms[J].Genomics,1990,7:524-530.
[49]  Thomas M R,Scott N S.Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as sequence-tagged sites (STSs)[J].Theoretical and Applied Genetics,1993,86:985-990.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133