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青枯菌诱导下花生基因组DNA表观遗传变化的MSAP分析

DOI: 10.7668/hbnxb.2015.02.017, PP. 93-99

Keywords: 花生,青枯菌,DNA甲基化,甲基化敏感扩增多态性

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Abstract:

以高感青枯病品种中花12号和高抗青枯病品种远杂9102为材料,调查青枯菌接种12h后花生基因组DNA的甲基化水平和变化模式。甲基化敏感扩增多态性(Methylation-sensitiveamplificationpolymorphism,MSAP)分析表明,正常生长的中花12号和远杂9102的基因组DNA甲基化比率均为35.1%,经青枯菌接种12h后,中花12号降低到31.3%,而远杂9102的基因组DNA甲基化比率则升高至37.2%。与对照相比,青枯菌接种12h胁迫下中花12号和远杂9102基因组DNA的甲基化和去甲基化分别为5.9%,12.4%,18.3%,11.9%。由此推测,高抗青枯菌的花生经青枯菌侵染后基因组DNA某些位点的甲基化状态发生变化而使基因表达发生了改变,产生或启动对青枯菌胁迫的能动应激机制。

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