Yao M Z,Zhang Y H,Lu W L,et al.Phytases:crystal structures,protein engineering and potential biotechnological applications[J].Journal of Applied Microbiology,2012,112(1):1-14.
Sreekrishna K,Brankamp R G,Kropp K E,et al.Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris[J].Gene,1997,190(1,SI):55-62.
Xiong A S,Yao Q H,Peng R H,et al.High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris [J].Applied Microbiology and Biotechnology,2006,72(5):1039-1047.
[11]
Yao M Z,Wang X,Wang W,et al.Improving the thermostability of Escherichia coli phytase,appA,by enhancement of glycosylation[J].Biotechnology Letters,2013,35(10):1669-1676.
[12]
Clare J J,Rayment F B,Ballantine S P,et al.High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene[J].Biotechnology,1991,9(5):455-460.
Xiong A S,Yao Q H,Peng R H,et al.Isolation,characterization,and molecular cloning of the cDNA encoding a novel phytase from Aspergillus Niger 113 and high expression in Pichia pastoris [J].Journal of Biochemistry and Molecular Biology,2004,37(3):282-291.
[17]
Stahl C H,Wilson D B,Lei X G.Comparison of extracellular Escherichia coli AppA phytases expressed in Streptomyces lividans and Pichia pastoris [J].Biotechnology Letters,2003,25(10):827-831.
Khatri N K,Hoffmann F.Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastori s[J].Biotechnology and Bioengineering,2006,93(5):871-879.
[22]
Mayson B E,Kilburn D G,Zamost B L,et al.Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica [J].Biotechnology and Bioengineering,2003,81(3):291-298.
