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牦牛CAPN4基因启动子区突变对胴体及肉质性状的影响

DOI: 10.7668/hbnxb.2015.02.006, PP. 28-34

Keywords: 牦牛,CAPN4基因,多态性,胴体及肉质性状

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Abstract:

为检测CAPN4基因在牦牛群体中的多态性,分析CAPN4基因启动子区突变对甘南牦牛胴体及肉质性状的影响,寻找与牦牛此类性状相关的分子遗传标记。采用PCR-SSCP技术检测830头甘南牦牛、大通牦牛和天祝白牦牛CAPN4基因启动子区突变并分析其多态性,运用SPSS19.0软件中的GLM模型分析基因突变对甘南牦牛胴体及肉质性状的影响。牦牛CAPN4基因启动子区存在a.-1222G>A的单碱基突变,检测到AA、AB和BB3种基因型。相关性分析结果表明,a.-1222G>A的突变对不同年龄甘南牦牛胴体和肉质性状有一定影响,其中4,6岁牦牛BB型个体熟肉率显著高于AA型或AB型(P<0.05);3,6岁牦牛AB型个体失水率显著高于BB型或AA型(P<0.05),而5岁牦牛BB型个体肌肉失水率显著高于AA型(P<0.05);3,5岁牦牛BB型个体的眼肌面积显著高于AA和AB型(P<0.05)。等位基因A对4,6岁牦牛熟肉率、各龄牦牛肌肉保水性和眼肌面积均存在不利影响;除5岁牦牛携带等位基因A个体的胴体重显著低于未携带者外,该突变对其他年龄段牦牛胴体重及全部牦牛个体的肌肉嫩度无显著影响。CAPN4基因启动子区a.-1222G>A突变影响不同年龄段甘南牦牛部分胴体及肉质性状,可作为甘南牦牛此类性状的遗传标记。

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