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大白菜育性相关蛋白差异表达

DOI: 10.7668/hbnxb.2015.02.023, PP. 128-131

Keywords: 大白菜,核基因雄性不育,iTRAQ,差异表达蛋白

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Abstract:

为揭示复等位基因遗传的大白菜雄性不育分子机制,通过基于质谱的蛋白质组学同位素相对标记与绝对定量技术技术(iTRAQ),开展了大白菜核基因雄性不育蛋白质组学研究,以找到不育与可育材料中差异表达蛋白,从蛋白水平来进一步揭示大白菜雄性不育的分子遗传机制。通过研究,共发现了358个差异蛋白,其中可育中上调表达的蛋白有226个,下调表达的蛋白有132个,GO分析结果表明,鉴定的蛋白质组数据具有较好的生物学功能覆盖范围。通过双向电泳验证,差异蛋白点差异特性与iTRAQ结果类似,表明iTRAQ用于差异蛋白分析结果可靠。

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