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大丽轮枝菌酵母双杂交cDNA文库的构建

DOI: 10.7668/hbnxb.2015.02.010, PP. 51-54

Keywords: 黄萎病,大丽轮枝菌,酵母双杂交文库,SMART技术

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Abstract:

为了分离克隆与植物互作的大丽轮枝菌致病相关蛋白基因,利用SMART技术构建了大丽轮枝菌酵母双杂交cDNA文库。结果表明,构建的文库滴度为5.2×107cfu/mL,库容为3.9×107cfu;文库cDNA插入片段长度主要分布在0.5~2.0kb,平均为1kb;文库重组率为96%。表明文库质量较好,为筛选与植物互作的大丽轮枝菌致病蛋白基因奠定了基础。

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