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白粉菌诱导的番茄叶片AD-cDNA文库的构建及初步分析

DOI: 10.7668/hbnxb.2015.02.009, PP. 47-50

Keywords: 番茄,番茄白粉菌,SMART技术,AD-cDNA文库

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Abstract:

为了研究番茄白粉菌与番茄相互作用的分子机制,以白粉菌诱导的番茄叶片为材料,利用SMART技术构建了番茄的AD-cDNA文库。该文库总库容量为3.77×1010cfu,重组率达到96%,插入片段长400~2000bp,平均长度约为1000bp。随机挑取50个克隆进行测序,利用生物信息学软件进行初步分析,表明获得的文库质量较高。

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